Proteomic response of LAP-activated RAW 264.7 macrophages to the anti-inflammatory property of fungal ergosterol |
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Authors: | Chia-Feng Kuo Chih-Hsuan HsiehWun-Yuan Lin |
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Affiliation: | Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 104, Taiwan |
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Abstract: | Ergosterol is the principal fungal sterol in edible mushrooms and yeast lees in wine. In this study, the immunoregulatory action of ergosterol was evaluated by a proteomic approach using two-dimensional gel electrophoresis to identify proteins with modified expression in LPS-challenged RAW 264.7 macrophages treated with ergosterol. By comparing the proteome signatures, alterations of multiple proteins were observed, including NF-κB cascade-related proteins, Rho family GTPase protein, and cytoskeleton-related proteins. The down-regulation of NF-κB cascade-associated proteins coincided with the inhibition of TNF-α production and COX-2 expression in the ergosterol-treated RAW 264.7 cells. Western blotting analysis indicated that ergosterol inhibits LPS-induced inflammation in RAW 264.7 macrophages by suppressing the NF-κB signalling pathway. |
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Keywords: | Ergosterol Macrophages Anti-inflammation |
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