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烟草VA菌根菌区系研究
引用本文:方宇澄,黄镇,刘延荣.烟草VA菌根菌区系研究[J].中国烟草学报,2000,6(4):26-30.
作者姓名:方宇澄  黄镇  刘延荣
作者单位:[1]中国科技大学烟草研究中心,合肥美菱大道121号,合肥230052 [2]山东农业大学烟草研究室,泰安271018
摘    要:从全国主要烟区12个省采集烟草根际土壤样本,以湿筛法分离出VA菌根菌的孢子。经接种烟草再次形成菌根并产生孢子后,用单一的新鲜孢子进行种的鉴定。由101份土样中分离出VA菌根菌294株。经过鉴定,定出种名的共33种,其中无梗囊霉属(Acaulospora)6种,巨孢囊霉属(Cdgaspora)3种,球囊霉属(Glomus)23种,盾孢囊霉属(Scutellospora)1种;有13种为国内新记录种。分析表明,在上述已鉴定的4个属中,球囊霉属分布最广;在33个种中,分布较广的有:A.laevis Gl.caledonium、Gl.caledonium、Gl.claroidum、Gl.clarum、Gl.diaphanum、Gl.etunicatum,、Gl.leptotichum 和Gl.mosseae。菌种的分布因土壤类型,土壤酸碱度和海拔高度而有一定差异。

关 键 词:烟草  VA菌根菌  区系分析  分离  鉴定

Study on VA mycorrhizal flora of tobacco (Nicotiana tobacum)
Fang Yuchung Huang Zhen Liu Yanrong University of Science and Technology of China,Hefei Shandong Agricultural University,Taian.Study on VA mycorrhizal flora of tobacco (Nicotiana tobacum)[J].Acta Tabacaria Sinica,2000,6(4):26-30.
Authors:Fang Yuchung Huang Zhen Liu Yanrong University of Science and Technology of China  Hefei Shandong Agricultural University  Taian
Affiliation:Fang Yuchung 1 Huang Zhen 1 Liu Yanrong 2 1 University of Science and Technology of China,Hefei 230052 2 Shandong Agricultural University,Taian 271018
Abstract:One hundred and one soil samples were collected from tobacco growing fields in 12 provinces across China. Spores of VA mycorrhizal fungi were separated by wet sieving method. Separated spores were inoculated on tobacco roots and cultured in sand tubes with low level nutrient solution. Individual spores were recollected and 294 isolates were obtained. Among the 294 isolates 33 species were identified, among them 6 species in genus Acaulospora, 3 in genus Gigaspora, 23 in genus Glomus and 1 in genus Scutellospora. Thirteen species were first recorded in China. It was showed that Glomus is the dominate genus and the species A. Laevis, Gl. caledonium, Gl. claroidum, Gl. clarum, Gl. diaphanum, Gl. etunicatum, Gl. leptotichum and Gl. mosseae distributed more extensively in the tobacco fields.
Keywords:VA mycorrhizal fungi  Flora  Tobacco  Identification of species
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