纳米磷灰石对肝癌癌基因表达的影响

研究纳米磷灰石体外对人肝癌细胞系Bel-7402的作用机理。应用均相共沉淀法室温下合成纳米磷灰石，用透射电镜和电位粒度仪进行表征；利用透射电镜、原位杂交细胞化学检测其对人肝癌细胞系Bel-7402 c-myc和p53基因表达的影响。纳米磷灰石呈均匀分散的针状颗粒．粒径范围在67．5～88．3nm之间。可使肝癌细胞体积增大，胞质空泡化，线粒体肿胀；部分细胞核内染色质分散，凝集在核膜周围，呈团块状，核膜间隙不等，胞浆溶解。抑制c-myc和增强p53基因的表达。纳米磷灰石在体外通过下调c-myc和上调p53基因的表达，致肝癌细胞胀亡。

Affection of Nano-apatite on the Expression of the Oncogene and Anti-oncogene in the Hepatocellular Carcinoma Cells

The mechanism of affection of the HAP nanoparticles on the human hepatocellular carcinoma in vitro was investigated. The nano-apatite was synthesized by using homogeneous precipitation method in room temperature and was characterized by using the transmission electron microscopy (TEM) and the zataplus; the affection on the expression of the c-myc and p53 gene in the human hepatocellular carcinoma was tested by the TEM and in situ hybridization cytochemistry. The nano-apatite was distributed homogenously in size and a plate-like shape. The average size ranged from 67.5 nm to 88.3 nm. The nano-apatite could increase the volume of the human hepatocellular carcinoma cells, make vacuolar in the cytoplasm and tumid in the mitochondria; chromatin in cell nucleus was dispersed partially and clumped around the nuclear membranes and was in the ball shapes. The interplace between the cell membranes was disparity and the cytoplasm was dissolved. The expression of the c-myc was inhibited but the p53 was enhanced. The nano-apatite made the oncosis of the human hepatocellular carcinoma cells by dropping the expression of the c-myc and increasing the expression of the p53 in vitro.