Aflatoxins in nuts assayed by immunological methods |
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Authors: | Joanna Leszczynska Urszula Kucharska Henryk Żegota |
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Affiliation: | (1) Institute of General Food Chemistry, Technical University, Stefanowskiego 4/10, PL-90-924 Lodz, Poland, PL;(2) Institute of Applied Radiation Chemistry, Technical University, Wroblewskiego 15, PL-93-590 Lodz, Poland e-mail: ahzegota@mitr.p.lodz.pl, PL |
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Abstract: | Different kinds of raw and processed nuts available in the local retail market were investigated for aflatoxin content. Total
aflatoxins and aflatoxin B1 content were determined by immunoaffinity chromatography with fluorescence detection after reaction with bromine solution
and by immunoenzymatic test kits. Of 29 investigated samples, 38% were contaminated. The total aflatoxin content in contaminated
samples was between 1.20 μg/kg for peanuts and 5.50 μg/kg for walnuts. The concentration of aflatoxin B1 found in contaminated samples was between 0.35 μg/kg for cashew nuts and 4.04 μg/kg for walnuts. The mean recovery of total
aflatoxins was 95% for the Ridascreen test and 92% for immunoaffinity chromatography with fluorescence detection. For aflatoxin
B1 the mean recovery was 84%.
Received: 4 March 1999 / Revised version: 17 May 1999 |
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Keywords: | Aflatoxins Nuts Enzyme immunoassay Immunoaffinity chromatography |
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