| MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO-PHASE PEG-POTASSIUM PHOSPHATE SYSTEM |
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| 引用本文： | 朱自强,李勉,梅乐和,韩兆熊. MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO-PHASE PEG-POTASSIUM PHOSPHATE SYSTEM[J]. 中国化学工程学报, 1996, 4(1): 19-27 |
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| 作者姓名： | 朱自强 李勉 梅乐和 韩兆熊 |
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| 作者单位： | Zhu Ziqiang Li MianMei Lehe Han ZhaoxiongDepartment of Chemical Engineering，Zhejiang University，Hangzhou 310027，China To whom correspondence should be addressed. |
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| 基金项目： | Supported by National Natural Science Foundation of China |
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| 摘 要： | Partition coefficients of Urokinase(UK)were measured in aqueous two-phase systems con-taining polyethylene glycol and potassium phosphate at 273.2K.Based on the Diamond-Hsu model,a modified expression was obtained for the correlation of enzyme partitioning in theabove-mentioned systems.Utilizing a modified form,the partitioning data of UK and heteroproteinwere correlated.The results show that the modified model is simple gives good precision.and will fa-cilitate engineering scale-up of aqueous two-phase systems for certain proteins purification.
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| 关 键 词： | partition coefficients urokinase aqueous two-phase system thermodynamic model |
| 收稿时间： | 1994-06-04 |
| 修稿时间： | 1995-3-31 |
MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO-PHASE PEG-POTASSIUM PHOSPHATE SYSTEM |
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| ZHU Ziqiang,LI Mian,MEI Lehe,HAN Zhaoxiong. MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO-PHASE PEG-POTASSIUM PHOSPHATE SYSTEM[J]. Chinese Journal of Chemical Engineering, 1996, 4(1): 19-27 |
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| Authors: | ZHU Ziqiang LI Mian MEI Lehe HAN Zhaoxiong |
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| Affiliation: | Department of Chemical Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | Partition coefficients of Urokinase (UK) were measured in aqueous two-phase systems containing polyethylene glycol and potassium phosphate at 273.2K. Based on the Diamond-Hsu model, a modified expression was obtained for the correlation of enzyme partitioning in the above mentioned systems. Utilizing a modified form, the partitioning data of UK and heteroprotein were correlated. The results show that the modified model is simple, gives good precision, and will facilitate engineering scale-up of aqueous two phase systems for certain proteins purification. |
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| Keywords: | partition coefficients urokinase aqueous two phase system thermodynamic model |
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