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Three-dimensional image formation in confocal microscopy |
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| Authors: | C J R Sheppard C J Cogswell |
| Abstract: | Three-dimensional (3-D) imaging in confocal microscopes is considered in terms of 3-D transfer functions. This leads to an explanation of axial imaging properties. The axial response was observed in both object-scanning and beam-scanning microscopes and the influence of off-axis examination investigated. By simple processing of multi-detector signals, imaging in both the axial and transverse directions can be improved. |
| Keywords: | Three-dimensional imaging confocal microscopy imaging theory scanning microscopy laser microscopy |