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Experimental ionization cross-sections of phosphorus and calcium by electron spectroscopic imaging
Authors:Y. M. Heng  G. T. Simon  M. Boublik  F. P. Ottensmeyer
Abstract:The absolute partial electron scattering cross-section for the phosphorus L2,3-shell ionization was measured by electron spectroscopic imaging using poliovirus as a primary standard. The equivalent calcium cross-section was obtained in relation to phosphorus using the stoichiometric ratio for these two elements in hydroxyapatite, Ca10(PO4)6(OH)2. At 80kcV, the partial cross-section of phosphorus was 2.26 times 10?20 and 2.68 × 10?20cm2/atom for poliovirus and hydroxyapatite, respectively, at 150eV loss for a 15-cV energy window and an acceptance angle of 15 mrad. Under the same conditions the calcium cross-section was 0.49 × 10?20 cm2/atom at 360 eV loss. The experimental values are slightly higher than the theoretical cross-sections calculated either by hydrogenic or Hartree—Slater approaches.
Keywords:Analytical electron microscopy  electron energy loss spectroscopy  electron spectroscopic imaging  elemental microanalysis  quantitative  ionization cross-section  near-edge fine-structure
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