| Abstract: | Endogenous ileal amino acid (free + bound) flow was determined in four 190-g male rats given a diet whose sole nitrogen source was enzyme hydrolysed casein and compared with that of six rats whose diet contained synthetic amino acids as the only nitrogen source but excluding alanine, glutamic acid and serine, and six rats fed a protein-free diet. The enzyme hydrolysed casein, a mixture of free amino acids and oligopeptides, was assumed to be completely absorbed in the small intestine of the rat. A further seven rats were given an enzyme hydrolysed casein-based diet and their ileal digesta were treated, post-collection, with perchloric acid to precipitate soluble proteins. The latter procedure removed the need to assume complete absorption of the casein nitrogen. The endogenous flow of amino acids for enzyme hydrolysed casein-fed rats was higher than that for the protein-free diet (P < 0.05) which did not differ from the synthetic amino acid diet for the flows of alanine, glutamic acid and serine. The high apparent digestibility of the synthetic amino acids not excluded from the diet indicated that their ileal excretions were mainly of endogenous origin. It would appear, therefore, that the protein deplete state per se does not influence endogenous amino acid flow at the terminal ileum of the growing rat, but there is a direct effect of small peptides on the net loss of endogenous amino acids from the small intestine. The estimates of endogenous amino acid flow obtained following the perchloric acid treatment of digesta were unrealistically low and thus inconclusive, but raised doubt as to the eficiency of perchloric acid in precipitating protein from rat ileal digesta. |
