A pH-controlled fed-batch process for dextransucrase production

A fed-batch culture of Leuconostoc mesenteroides has been operated from an initial volume of 4 dm³ using a sucrose/NaOH solution feed on demand with pH control. An enzyme concentration of greater than 300 DSU cm^?3 was achieved in 15 h. A yield of 0.107 kg cells kg^?1 sucrose feed was determined. The enzyme production was growth associated at 2.67 × 10⁷ DSU kg^?1 cells produced. Growth was shown to be linear and attributed to an unknown conservative trace nutrient limitation associated with the yeast extract.