温胆汤治疗肿瘤相关胃肠道反应的机制研究

目的：观察温胆汤对荷瘤小鼠的胃肠道5-羟色胺和嗜铬细胞分泌的影响。方法皮下注射 Colon-26腺瘤建立小鼠荷瘤模型。将32只小鼠随机分为温胆汤组和荷瘤对照组。在第7、14天用高效液相色谱法测定小肠中5-羟色胺的含量，用免疫组化法检测嗜铬细胞的密度。并观察每组小鼠的摄食量。结果正常小鼠第1周和第2周小肠的5-羟色胺含量分别为（1．57±0．09）μg·g-1和（1．54±0．11）μg·g-1，而接种肿瘤细胞后的小鼠分别为（2．93±0．17）μg·g-1和（3．99±0．32）μg·g-1，显著高于正常小鼠。而温胆汤组则分别降到（2．03±0．17）μg· g-1和（3．05±0．17）μg·g-1，显著低于荷瘤对照组，2组比较差异有统计学意义（P＜0．01）。免疫组化显示小肠中嗜铬细胞在肿瘤细胞移植后的第1周和第2周显著增加，而服用温胆汤的小鼠小肠嗜铬细胞数量明显降低，2组比较差异有统计学意义（P＜0．01）。在第14天，荷瘤组小鼠和温胆汤组小鼠的平均食量分别为（0．70±0．12）g和（1．12±0．04）g，2组比较差异有统计学意义（P＜0．01）。结论温胆汤可以通过减少肿瘤患者肠道嗜铬细胞数量和5-羟色胺含量而减轻肿瘤相关胃肠道反应。

Mechanisms for Effect of Wendan Decoction on Tumor-Associated Gastrointestinal Tract Reaction

Objective To observe the effects of Wendan decoction on serotonin production and chromaffin cell secretion in the gastrointestinal tract in tumor-bearing mice.Methods Colon-26 carcinoma cells were inj ected subcutaneously into mice to establish the tumor-bearing model. Thirty-two tumor-bearing mice were randomly divided into Wendan decoction treatment group and tumor-bearing group.The content of serotonin in small intestine was determined by HPLC and the number of enterochromaffin cells was measured by immunochemistry at 7 and 14 days af-ter inoculation of tumor cells.In addition,food intake was measured.Results At 7 and 14 days after inoculation of tumor cells,the contents of serotonin in small intestine were,respectively,（1 .5 7 ± 0.09）and （1.54±0.11）μg·g-1 in normal mice,（2.93±0.17）and （3.99±0.32）μg·g-1 in tumor-bearing group,and（2.03±0.17）and （3.05±0.17）μg·g-1 in Wendan decoction treat-ment group.The differences in serotonin content were significant among the three groups （P〈0.01）.Compared normal controls,the number of enterochromaffin cells significantly increased in tumor-bearing group at 7 and 14 days after inoculation of tumor cells（P〈0.01）.However,Wen-dan decoction treatment significantly decreased the number of enterochromaffin cells compared with tumor-bearing group （P〈0.01 ）.Moreover,Wendan decoction treatment significantly de-creased the food intake compared with tumor-bearing group at 14 days after inoculation of tumor cells（P〈0.01）.Conclusion Wendan decoction treatment can alleviate tumor-associated gastroin-testinal tract reaction through reducing the number of enterochromaffin cells and the content of serotonin.