Minimum inhibitory concentrations of antimicrobials against micro-organisms related to citrus juice |
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| Affiliation: | 1. Department of Urban Engineering, Graduate School of Engineering, the University of Tokyo, Tokyo 113-8654, Japan;2. Department of Food, Life and Environmental Sciences, Faculty of Agriculture, Yamagata University, Yamagata 997-8555, Japan;3. Department of Civil and Environmental Engineering, Ehime University, Ehime 790-8577, Japan;4. Institute for the Advanced Study of Sustainability, the United Nations University, Tokyo 150-8925, Japan;5. Todai Institutes for Advanced Study (TODIAS), Integrated Research System for Sustainability Science (IR3S), The University of Tokyo, Tokyo 113-8654, Japan;1. Parasitology Laboratory, Water Pollution Research Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, 12622 Giza, Egypt;2. Department of Biology, Faculty of Science, University of Tabuk, P.O. Box 741, Tabuk 71491, Saudi Arabia;3. Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;4. Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan 11795, Egypt |
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| Abstract: | Minimum inhibitory concentrations (MIC) of various cleaner or sanitizer compounds were determined by a Spiral Gradient Endpoint agar diffusion method against four micro-organisms associated with spoilage of orange juice (Lactobacillus plantarum,Leuconostoc mesenteroides,Gluconobacter oxydans, andSaccharomyces cerevisiae) and against eight unidentified micro-organisms isolated from citrus fruit surfaces (two yeasts and six bacteria). Survivor curve testing was conducted on the four known micro-organisms. Compounds that were effective at the lowest concentrations were chlorine dioxide, the iodophor and quaternary ammonium compound. Dimethyldicarbonate, hypochlorite, peracetic acid and a phosphoric acid anionic compound also possessed substantial antimicrobial activity. Citric and lactic acids andd-limonene were less effective as antimicrobial compounds. |
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