| 重组人乳铁蛋白致敏性的初步研究 |
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| 引用本文： | 刘珊,陈惠芳,王小丹,冯晓莲,李晨汐,徐海滨. 重组人乳铁蛋白致敏性的初步研究[J]. 中国食品卫生杂志, 2013, 25(2): 107-112 |
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| 作者姓名： | 刘珊 陈惠芳 王小丹 冯晓莲 李晨汐 徐海滨 |
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| 作者单位： | 1. 国家食品安全风险评估中心,北京 100021;中国疾病预防控制中心营养与食品安全所,北京 100021
2. 广州医学院第二附属医院/呼吸疾病国家重点实验室/广州市过敏反应与临床免疫重点实验室,广东广州,510260 |
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| 基金项目： | 转基因生物新品种培育科技重大专项 |
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| 摘 要： | 目的 初步评价牛乳腺生物反应器表达的重组人乳铁蛋白(rhLf)的致敏性.方法 通过生物信息学分析和消化稳定性试验了解rhLf致敏的可能性.结果 生物信息学分析显示rhLf与已知致敏原乳转铁蛋白和卵转铁蛋白有序列同源性,与Asp f2、Ole e 10和Ole e9存在结构相似性.消化稳定性试验显示rhLf在胃液中易被消化成小片段,在肠液中不易被消化.结论 rhLf具有一定的潜在致敏可能性.
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| 关 键 词： | 重组蛋白 人乳铁蛋白 转基因食品 致敏性 食品安全 |
| 收稿时间： | 2012-09-26 |
| 修稿时间： | 2013-01-22 |
Preliminary assessment of recombinant human lactoferrin for allergenic potential |
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| Liu Shan,Chen Huifang,Wang Xiaodan,Feng Xiaolian,Li Chenxi and Xu Haibin. Preliminary assessment of recombinant human lactoferrin for allergenic potential[J]. Chinese Journal of Food Hygiene, 2013, 25(2): 107-112 |
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| Authors: | Liu Shan Chen Huifang Wang Xiaodan Feng Xiaolian Li Chenxi Xu Haibin |
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| Affiliation: | China National Center for Food Safety Risk Assessment |
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| Abstract: | To evaluate the allergenic potential of recombinant human lactoferrin (rhLf) expressed by genetically modified cow in milk.Methods Bioinformatics searches and digestibility assay were conducted to investigate the allergenic potential of rhLf. [WT5"HZ]Results Bioinformatics searches showed that there was high-level similarity between rhLf and some known allergens, such as lactotransferrin and ovotransferrin in amino acid sequence, and Asp f 2, Ole e 10and Ole e 9in structure. Digestibility assay showed that rhLf broke down into small fragment rapidly during gastric fluid digestion, while it was resistant to intestinal fluid digestion.ConclusionRhLf has allergenic potential. |
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