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Identification of Sex Pheromone of Adzuki Bean Borer,Ostrinia scapulalis
Authors:Yongping Huang  Sadahiro Tatsuki  Choong-Gon Kim  Sugihiko Hoshizaki  Yutaka Yoshiyasu  Hiroshi Honda  Yukio Ishikawa
Affiliation:(1) Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan;(2) Laboratory of Entomology, Kyoto Prefectural University, Sakyo-ku, Kyoto, 606, Japan;(3) Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, 305, Japan
Abstract:By means of gas chromatography with electroantennographic detection (GC-EAD), gas chromatography–mass spectrometry (GC-MS), and a series of bioassays, (Z)-11-tetradecenyl acetate (Z11–14:OAc) and (E)-11-tetradecenyl acetate (E11–14:OAc) at a ratio of 100:3 were identified as the female sex pheromone of the adzuki bean borer,Ostrinia scapulalis. The average amounts ofZ11–14: OAc andE11–14:OAc in a single sex pheromone gland were 6.6 ± 2.4 ng and 0.2 ± 0.1 ng, respectively. In a wind-tunnel bioassay, the binary blend ofZ11- andE11–14:OAc elicited almost the same male behavioral responses as did virgin females and sex pheromone gland extract. In field trapping experiments, rubber septa impregnated with the binary blend (50 mgrg/septum) attracted more males than virgin females. The sex pheromone ofO. scapulalis thus turned out to be similar to that of theZ-type European corn borer,O. nubilalis, in both components and their ratio.
Keywords:Ostrinia scapulalis  sex pheromone  (Z)-11-tetradecenyl acetate  (E)-11-tetradecenyl acetate  GC-EAD  field trapping
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