| 氢离子敏场效应管酶免疫传感器的研制 |
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| 引用本文： | 方玮 任恕. 氢离子敏场效应管酶免疫传感器的研制[J]. 传感器与微系统, 1995, 0(3): 23-27 |
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| 作者姓名： | 方玮 任恕 |
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| 作者单位： | 同济医科大学 |
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| 摘 要： | 介绍了以氢离子敏场效应管为基体电极，以碱性磷酸酶抗碱性磷酸酶抗体为例，在方法学上实现了电化学测量的一种酶免疫传感器．本生物传感器的响应时间达１８ｓ，标准曲线线性范围为７．８×１０（－２）μｇ／ｍｌ～０．６３μｇ／ｍｌ。另外，还讨论了适宜测量条件、抗体膜的寿命、抗原抗体复合物的寿命、抗原抗体复合物的分离及用不同抗体膜测量的重复性等。
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| 关 键 词： | 酶免疫传感器，氢离子敏场效应管，碱性磷酸酶抗碱性磷酸酶抗体 |
The Study of an Enzyme Immunosensor Based on Hydrogen Ion Sensitive Field Effect Transistor (H￣+-ISFET) |
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| Fang Wei, Ren Shu, Zhu Yexiang. The Study of an Enzyme Immunosensor Based on Hydrogen Ion Sensitive Field Effect Transistor (H￣+-ISFET)[J]. Transducer and Microsystem Technology, 1995, 0(3): 23-27 |
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| Authors: | Fang Wei Ren Shu Zhu Yexiang |
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| Affiliation: | Tongji Medical University |
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| Abstract: | An enzyme immunosensor based on a pair of hydrogen ion sensitive field effect transistors (H+-ISFET) for the determination of alkaline phocphatase anti-alkaline-phosphatase antibody (APAAP) is reported. A linear relationship was obtained between the respoase peak and the logarithm of APAAP concentration from 7.8×10(-2)μg/ml to 0.63μg/ml. The response the time was only 18s.The appropriate condition for the measurement, the reversibility of the measurements with different membranes and the methods to dissociate the GAM-APAAP immunocomplexes are also discussed. |
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| Keywords: | Enzyme immunosensor H+-ISEFT APAAP |
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