| 本底校正褪色光度法测定食品中还原型维生素C |
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| 引用本文： | 徐茂军,项雷文,包开洪.本底校正褪色光度法测定食品中还原型维生素C[J].食品与发酵工业,2001,27(4):35-38. |
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| 作者姓名： | 徐茂军 项雷文 包开洪 |
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| 作者单位： | 杭州商学院食品科学工程学院, |
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| 摘 要： | 还原型维生素C能将Fe3+定量还原 ,通过测定反应中所消耗的Fe3+量 ,即可间接测定样品中还原型维生素C含量。利用KSCN和罗丹明B能与Fe3+形成离子型缔合物的特性和表面活性剂吐温 80 (Tween 80 )的胶束增溶作用 ,使还原型维生素C的测定灵敏度显著提高。利用Cu2 +能将样品中还原型维生素C选择性氧化的特性 ,通过对食品样品的Cu2 +预氧化处理 ,实现对有色样品本底吸收的快速校正 ,建立了一种本底校正褪色光度法测定食品中还原型维生素C的新方法。应用本文方法测定了多种食品样品中还原型维生素C的含量 ,取得了满意的结果
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| 关 键 词： | 还原型维生素C 测定 褪色光度法 本底校正 |
| 修稿时间： | 2000年8月11日 |
Determination of Ascorbic
Acid in Foods by Using Background Correction Decoloring Spectrophotometry |
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| Xu Maojun,Xiang Leiwen,Bao Kaihong.Determination of Ascorbic
Acid in Foods by Using Background Correction Decoloring Spectrophotometry[J].Food and Fermentation Industries,2001,27(4):35-38. |
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| Authors: | Xu Maojun Xiang Leiwen Bao Kaihong |
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| Abstract: | Ascorbic acid was determined indirectly based on the oxidation-reduction reaction between ascorbic acid and Fe3+. The sensitivity was increased with the production of the complex of thiocyanate、Fe2+ and rhodamine B in the presence of Tween-80. The background absorbance of color samples was corrected by Cu2+ preoxidation of the samples. A new method for the determination of ascorbic acid using background decolor spectrophotometry was established basing on above results and was applied to analysis of ascorbic acid in many fruits and vegetables with satisfactory results. |
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| Keywords: | ascorbic acid determination decoloring spectrophotometry background correction |
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