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| 水酶法提取光皮树油的研究 | |
| 引用本文： | 申爱荣,谭著明,李昌珠,肖志红,陈金良,黄玉芳,蒋丽娟.水酶法提取光皮树油的研究[J].中国油脂,2010,35(3). |
| 作者姓名： | 申爱荣 谭著明 李昌珠 肖志红 陈金良 黄玉芳 蒋丽娟 |
| 作者单位： | 1. 湖南省林业科学院,长沙,410004 2. 河南工业大学,郑州,450001 3. 长沙创林有限公司,长沙,410004 4. 中南林业科技大学,长沙,410004 |
| 基金项目： | 国家科技部科技支撑项目《生物柴油绿色高效催化剂的研究》，生物柴油清洁生产工艺研究与示范 |
| 摘 要： | 从市售的木聚糖酶、淀粉酶、纤维素酶、中性蛋白酶、复合植物水解酶、果胶酶中筛选提取光皮树油的水解酶,实验结果得到纤维素酶对光皮树果实的提油作用最强,其提油率达65.29%,复合植物水解酶次之,为64.05%,木聚糖酶效果最差,仅为54.47%。通过单因素实验得到纤维素酶提取光皮树油的最适工艺条件为:酶解pH 5.8,料液比1∶3,酶加量2.5%,酶解温度40℃,酶解时间4h。该条件下,油的乳化率低,提出来的基本上是清油,且提油率达76.64%。 |
| 关 键 词： | 光皮树油 水酶法 纤维素酶 筛选 |
Aqueous enzymatic extraction of Cornus wilsoniana fruit oil |
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| SHEN Airong,TAN Zhuming,LI Changzhu,XIAO Zhihong,CHEN Jinliang,HUANG Yufang,JIANG Lijuan.Aqueous enzymatic extraction of Cornus wilsoniana fruit oil[J].China Oils and Fats,2010,35(3). | |
| Authors: | SHEN Airong TAN Zhuming LI Changzhu XIAO Zhihong CHEN Jinliang HUANG Yufang JIANG Lijuan |
| Abstract: | The optimum enzyme of extracting oil from Cornus wilsoniana fruit was selected from xylanase,amylase,cellulase,neutral protease,composite plant hydrolytic enzymes and pectinase.The results showed that the oil extraction rate (65.29%) with cellulase was the highest among the six kinds of enzyme,and the oil exraction rate (64.05%) with composite plant hydrolytic enzymes was the second,the lowest oil extraction rate was 54.47% only by xylanase.By single factor test,the optimum conditions with cellulase was obtained as follows: pH 5.8,solid-liquid ratio 1∶ 3,enzyme dosage 2.5%,reaction temperature 40 ℃,reaction time 4 h.Under these conditions,the oil extraction rate reached 76.64%. |
| Keywords: | Cornus wilsoniana fruit oil aqueous enzymatic extraction cellulase selection |
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