葛根黄酮提取工艺优化

摘要：为研究葛根黄酮的提取工艺，对多个影响因素进行了单因素考察，并选择乙醇质量分数、提取时间、液固比与提取温度4个因素采用响应面法进行了优化，建立了数学模型并进行了方差分析。结果表明，所得模型能很好地描述提取率与各因素之间的关系，黄酮提取的最佳工艺为：乙醇质量分数63．9％、提取时间138．0min、液固比11．3mL／g、提取温度72．9℃、1次提取；提取时溶液pH值不需调整；乙醇回收循环使用可行。采用该工艺条件，黄酮理论提取率可达到1842．6ug／g，实际为1821．3ug／g。

Optimum extracting technology of flavonoids from radix pueraria

The muhiple influence factors were investigated through the single factor for researching the extraction technology of flavonoids from radix pueraria. Four factors of quality fraction of ethanol, extraction time, liquid-solid ratio and extraction temperature were chosen to optimize with response surface methodology（RSM）. A mathematical model was established and the analysis of variance was carried out. The result shows that the obtained model is significant and the optimum extraction conditions are as follows： 63.9 %0 of quality fraction of ethanol, 138.0 min of extraction time, 11.3 mL/g of liquid-solid ratio, 72.9 ~C of extraction temperatui＇e and one time extraction; when extracting the pH value of solution does not need to adjust; the recycle use of ethanol is feasible. Using the process conditions, the theoretical extraction yield of flavonoids is 1 842.6 /~g/g; the practical extraction yield is 1 821.3 ~g/g.