| 枯草杆菌溶栓酶的恒溶氧发酵研究 |
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| 引用本文： | 韩润林,张小勇,李佐虎,张建安.枯草杆菌溶栓酶的恒溶氧发酵研究[J].化工学报,2000,51(Z1):268-271. |
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| 作者姓名： | 韩润林 张小勇 李佐虎 张建安 |
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| 作者单位： | 中国科学院化工冶金研究所生化工程国家重点实验室!北京 100080,中国科学院化工冶金研究所生化工程国家重点实验室!北京 100080,中国科学院化工冶金研究所生化工程国家重点实验室!北京 100080,清华大学化学工程系!北京100084 |
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| 摘 要： | 本文以枯草杆菌（Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｉｓ）ＨＬ－９８６为试验菌株，对其恒溶氧发酵生产溶栓酶的的过程进行了研究，并与恒通气速率发酵作了比较。结果表明：与恒通气速率发酵相比，恒溶氧发酵可使溶栓酶生产能力提高３８．８％，在２２ｈ时溶栓酶活力可达 ９３０ＩＵ／ｍｌ，最佳条件是在发酵初期控制恒溶氧１５％。
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PRODUCTION OF THROMBOLYTIC ENZYME BY DO-STAT BATCH FERMENTATION EMPLOYING Bacillus subtilis Hl-986 |
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| Han Runlin,Zhang Xiaoyong and Li Zuohu,Zhang Jianan.PRODUCTION OF THROMBOLYTIC ENZYME BY DO-STAT BATCH FERMENTATION EMPLOYING Bacillus subtilis Hl-986[J].Journal of Chemical Industry and Engineering(China),2000,51(Z1):268-271. |
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| Authors: | Han Runlin Zhang Xiaoyong and Li Zuohu Zhang Jianan |
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| Affiliation: | Han Runlin,Zhang Xiaoyong and Li Zuohu,Zhang Jianan (Inst. Chem. Metall.,Chinese Academy of Sciences,Beijing ) (Dept. Of Chem.Eng.,Tsinghua Vniv.,Beijing ) |
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| Abstract: | DO-stat batch fermentation of a thrombolytic enzyme from Bacillus subtilis HL-986 was studied. The thrombolytic enzyme yield reached 930IU/ml by DO-stat fermentation, and was increased 38.8% compared with constant aeration rate. The optimum DO-stat level is 15% controlled in the beginning of fermentstion. |
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