| 抗菌肽cecropin P1在大肠杆菌中的融合表达及活性测定 |
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| 引用本文： | 翟琼,叶江,莫剑,张惠展.抗菌肽cecropin P1在大肠杆菌中的融合表达及活性测定[J].食品与药品,2011,13(1):1-5. |
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| 作者姓名： | 翟琼 叶江 莫剑 张惠展 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 目的 在大肠杆菌中融合表达抗菌肽cecropin P1.方法 根据抗菌肽ceeropin P1的氨基酸序列,依照大肠杆菌密码子的偏爱性,采SOE技术合成cecropin P1的编码基因;将其克隆至表达载体pET-32a(+)上,经IPTG诱导表达,超声上清液用Ni·NTA亲和层析初步纯化,经肠激酶切割后,用薄层平皿琼脂...
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| 关 键 词： | cecropin P1 融合表达 Ni-NTA亲和层析 肠激酶 抑菌活性 |
Fusion Expression of Cecropin P1 in Escherichia Coli and Its Antimicrobial Activity Analysis |
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| ZHAI Qiong,YE Jiang,MO Jian,ZHANG Hui-zhan.Fusion Expression of Cecropin P1 in Escherichia Coli and Its Antimicrobial Activity Analysis[J].Food and Drug,2011,13(1):1-5. |
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| Authors: | ZHAI Qiong YE Jiang MO Jian ZHANG Hui-zhan |
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| Affiliation: | ZHAI Qiong,YE Jiang,MO Jian,ZHANG Hui-zhan (State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China) |
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| Abstract: | Objective To achieve the fusion expression of cecropin P1 in E.coli.Methods According to the biased coden used in E.coli,the gene sequence encoding cecropin P1 was designed.The gene fragment of cecropin P1 was first obtained through gene SOEing;then the DNA sequence was inserted into pET-32a(+) vector.After IPTG induction and Ni-NTA affinity chromatography,the fusion protein was cleaved by recombinant enterokinase.The activity of recombinant cecropin P1 was determined by agarose diffusion assay on thin-laye... |
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| Keywords: | cecropin P1 |
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