Pleurotusostreatus and Agaricus subrufescens: investigation of chemical composition and antioxidant properties of these mushrooms cultivated with different handmade and commercial supplements |
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| Authors: | Anna Beatriz Sabino Ferrari George Azevedo de Oliveira Helena Mannochio Russo Luiza de Carvalho Bertozo Vanderlan da Silva Bolzani Diego Cunha Zied Valdecir Farias Ximenes Maria Luiza Zeraik |
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| Affiliation: | 1. Laboratory of Phytochemistry and Biomolecules (LabFitoBio), Department of Chemistry, State University of Londrina (UEL), Rodovia Celso Garcia Cid | Pr 445 Km 380, Londrina, PR, 86051-990 Brazil;2. NuBBE, Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Av. Prof. Francisco Degni, 55, Araraquara, SP, 14800-060 Brazil;3. Departament of Chemistry, Faculty of Sciences, São Paulo State University (UNESP), Av Eng° Luiz Edmundo Carrijo Coube, S/N, Bauru, SP, 17033-360 Brazil;4. Faculty of Agrarian and Technological Sciences, São Paulo State University (UNESP), Rod. Cmte João Ribeiro de Barros, km 651, Dracena, SP, 17900-000 Brazil |
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| Abstract: | In this study, the chemical composition, total phenolic content and antioxidant activity (DPPH, ORAC and FRAP assays) of A. subrufescens and P. ostreatus, cultivated with handmade and commercials supplements, were compared. Additionally, the compounds ergosterol, saccharopine, and hexitol were identified in A. subrufescens by HPLC-MS/MS. The antioxidant compound p-coumaric acid and dihexoses was found in both mushroom species. A. subrufescens presented higher total phenolic content (73.8 ± 0.6 mg GAE 100 g−1) and antioxidant activity than P. ostreatus (16.6 ± 0.5 mg GAE 100 g−1). The handmade supplement based on the waste of noble grains presented statistically similar phenolic content to the mushrooms cultivated with commercial ones Spawn Mate II SE (86.1 ± 1.4 and 92.9 ± 0.3 mg GAE 100 g−1, respectively). Therefore, the results support the use of handmade supplements based on agro-wastes as a viable alternative to the use of high-cost commercial ones. |
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| Keywords: | Agaricus subrufescens functional food HPLC-MS mass spectrometry MS Pleurotus ostreatus supplementation |
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