|
|||||
|
|
Determination of aflatoxins,deoxynivalenol, ochratoxin A and zearalenone in organic wheat flour under different storage conditions |
|
| Authors: | Loredana Annunziata Maria Schirone Pierina Visciano Guido Campana Maria Rosaria De Massis Giacomo Migliorati |
| Affiliation: | 1. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Unit of Bromatologia e Residui, Via Campo Boario, Teramo, 64100 Italy Contribution: Investigation (lead), Methodology (lead), Writing - original draft (lead), Writing - review & editing (lead);2. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, Teramo, 64100 Italy Contribution: Conceptualization (lead), Writing - original draft (lead), Writing - review & editing (lead);3. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, Teramo, 64100 Italy;4. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Unit of Bromatologia e Residui, Via Campo Boario, Teramo, 64100 Italy Contribution: Formal analysis (lead), Methodology (supporting);5. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Unit of Bromatologia e Residui, Via Campo Boario, Teramo, 64100 Italy Contribution: Formal analysis (supporting), Investigation (supporting);6. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Unit of Bromatologia e Residui, Via Campo Boario, Teramo, 64100 Italy Contribution: Investigation (supporting), Writing - review & editing (supporting) |
| Abstract: | Samples of flour derived from an organic wheat seed cultivated in the region Abruzzo (Central Italy) were examined for the detection of aflatoxins, deoxynivalenol, ochratoxin A and zearalenone after storage in the presence of oxygen or under vacuum, for one year. They were analysed by high-performance liquid chromatography with fluorescence detection for aflatoxins, ochratoxin A and zearalenone and with diode array detector for deoxynivalenol. An immunoaffinity column clean-up was applied for all the compounds, except for aflatoxin determination, which required a solid-phase extraction. Recoveries (range: 76–107%) and within-laboratory reproducibility (RSD% < 10.3) met the performance criteria of Commission Regulation (EU) No. 519/2014 amending Commission Regulation (EC) No. 401/2006 for the detection of mycotoxins in foodstuffs. Analytes were below the detection limit of the method, and this result was probably due to the quality of organic seed and good agricultural practices performed for the prevention of mycotoxin formation. |
| Keywords: | Flour HPLC mycotoxins organic wheat |