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Evaluation of antibacterial protein with antioxidant activity from Rahnella aquatilis L103 and its effect on beef during refrigerated storage |
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| Authors: | Huan-yu Tao Qin Hu Yi Wu Peng Han Xiaobo Liang |
| Affiliation: | 1. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan, 650500 China
?These authors contributed equally to this work. Contribution: Project administration (lead);2. Department of Diabetes, The First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, 650500 China ?These authors contributed equally to this work. Contribution: Writing - review & editing (equal);3. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan, 650500 China Contribution: Formal analysis (equal);4. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan, 650500 China;5. Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan, 650500 China Contribution: Project administration (equal) |
| Abstract: | A strain, Rahnella aquatilis L103, which was isolated from the soil around the roots of mushrooms, produced antibacterial protein though fermentation. This protein has broad antibacterial spectrum towards gram-positive and gram-negative bacteria. The protein has a significant antioxidant capacity on scavenging ABTS+· (82.5%, 120 μg mL?1), DPPH· (64.1%, 600 μg mL?1) and OH· (60.1%, 750 μg mL?1) as well. The protein was considered to be safe within concentration of 150 μg mL?1 as shown by MTT results and was applied to beef refrigerated preservation. The treatment group presented lower total viable counts (TVC), total volatile basic nitrogen (TVB-N), 2-thiobarbituric acid (TBA), meanwhile, higher sensory score and longer shelf life (2 days) compared with the control group (P < 0.05). The results indicated that the protein under safe concentration was beneficial to beef preservation. |
| Keywords: | Antibacterial protein antioxidant capacity beef preservation broad antibacterial spectrum Rahnella aquatilis |