Antibiofilm effects of pomegranate peel extracts against B. cereus,B. subtilis,and E. faecalis |
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Authors: | Merve Balaban Cansel Koc Taner Sar Meltem Yesilcimen Akbas |
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Affiliation: | 1. Department of Molecular Biology and Genetics, Gebze Technical University, Gebze-Kocaeli, 41400 Turkey;2. Swedish Centre for Resource Recovery, University of Borås, Borås, 501 90 Sweden |
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Abstract: | In this study, the antibacterial and antibiofilm properties of pomegranate (Punica granatum L.) extracts (PPLs) prepared by 10 g of pomegranate peels (PPL10) and 100 mL of different solvents (ethanol, methanol, and their acid combinations, and water) were investigated as sources of bioactive compounds against food-related bacteria. The antimicrobial activities of the extracts were measured by using agar well diffusion assay. The acid-treated extracts exhibited the highest antimicrobial activities (31–34 mm). The biofilm formations were assessed by using a microplate reader (570 nm) after crystal violet staining. The prevention and removal of bacterial biofilms were also evaluated by using extracts prepared by 5 g of initial pomegranate peel (PPL5) with the different solvents or 200 ppm chlorine solution. All PPL5 and PPL10 extracts diluted at different ratios inhibited and removed biofilms, and the highest antibiofilm effects were up to 80% by acid-treated extracts. In addition, PPL5s and PPL10s were as effective as or more effective than chlorine for the prevention of biofilms. Therefore, from an economical perspective the PPL5s could represent promising candidates as natural antibiofilm agents for food industry. |
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Keywords: | Antibiofilm antimicrobial bioactive compounds extraction food waste pomegranate peel |
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