Use of nanoliposome loaded with chitosan-epigallocatechin gallate conjugate for shelf-life extension of refrigerated Asian sea bass (Lates calcarifer) slices |
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Authors: | Ajay Mittal Avtar Singh Soottawat Benjakul |
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Affiliation: | International Center of Excellence in Seafood Science and Innovation (ICE-SSI), Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110 Thailand |
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Abstract: | Treatments of chitosan-epigallocatechin gallate (CE) conjugate nanoliposome (CELP) and unencapsulated CE conjugate (UNCE) at different concentrations (0.025 and 0.05 g 100 g−1) on quality and shelf-life of Asian sea bass slices (ASB-S) kept at 4 °C were studied. Total viable count of ASB-S treated with 0.05 g 100 g−1 CELP (SB-CELP-0.05) was less than permissible limit (6 log CFUg−1 sample) within 15 days. Moreover, SB-CELP-0.05 sample had the lowest pathogenic and spoilage bacterial count than remaining samples during the storage (P < 0.05). Lower thiobarbituric acid reactive substances and peroxide values were obtained for SB-CELP-0.05 sample than those treated with 0.05 g 100 g−1 UNCE (SB-UNCE-0.05) at the end of storage (P < 0.05). The result was also advocated by higher polyunsaturated fatty acid content of SB-CELP-0.05 than SB-UNCE-0.05 (P < 0.05). Therefore, CELP efficiently extended ASB-S shelf-life with high consumer acceptability at 4 °C for minimum 12 days, whereas control had the shelf-life less than 6 days. |
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Keywords: | Chitosan-epigallocatechin gallate conjugate nanoliposome shelf-life Asian sea bass |
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