Deciphering the role of selenium-enriched rice protein hydrolysates in the regulation of Pb2+-induced cytotoxicity: an in vitro Caco-2 cell model study |
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| Authors: | Yiqing Zhu Jian Ding Yi Shi Yong Fang Peng Li Fengjiao Fan Jian Wu Qiuhui Hu |
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| Affiliation: | 1. College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023 China
The first and second authors contributed equally to this work.;2. College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023 China |
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| Abstract: | Lead (Pb), a well-known toxic metal, has a potential of adverse effects on human health. Selenium (Se) can reduce the toxicity caused by heavy metals. In this study, we investigated the protection mechanism of the purified Se-enriched rice protein hydrolysates (SPHs-2) on the Pb2+-induced cytotoxicity. A Caco-2 cell model was established to characterise the effect of SPHs-2 on Pb2+ absorption in the simulated small intestine. Results showed that 1000 μg mL?1 of SPHs-2 treatment could significantly increase cell viability by 19.43% in Pb2+-treated Caco-2 cells (P < 0.05). Furthermore, the absorption of Pb2+ was successfully retarded by incorporating SPHs-2. Interestingly, selenomethionine (SeMet) was identified as a major Se species in SPHs-2 by HPLC-ICP-MS, and SeMet played a crucial role in regulating Pb2+ intestinal absorption via forming complexes. In light of this, SPHs-2 may serve as a potential Pb2+-chelating peptide for developing novel functional food and protecting human health. |
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| Keywords: | Absorption caco-2 cell cytotoxicity lead selenium-enriched rice protein hydrolysates |
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