| HPLC测定盐酸奥洛他定滴眼液中苯扎氯铵的含量 |
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| 引用本文： | 韩文芳,韩保萍,朱启登,李晓强,邢贞琦.HPLC测定盐酸奥洛他定滴眼液中苯扎氯铵的含量[J].山东食品科技,2010(11). |
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| 作者姓名： | 韩文芳 韩保萍 朱启登 李晓强 邢贞琦 |
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| 作者单位： | 山东博士伦福瑞达制药有限公司 |
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| 摘 要： | 目的建立HPLC测定盐酸奥洛他定滴眼液中苯扎氯铵含量的方法。方法以氰基键合硅胶为填充剂;乙腈-水-三乙胺为440∶560∶1,用磷酸调pH 3.4为流动相,流速1.0 mL/min;检测波长210 nm。结果苯扎氯铵在0.05～0.15 mg/mL范围内呈良好的线性关系(r=0.999 8);平均回收率99.7%(n=9,RSD=0.69%)。结论此方法简便灵敏准确,重复性好,可用于测定盐酸奥洛他定滴眼液中苯扎氯铵的含量。
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| 关 键 词： | 盐酸奥洛他定滴眼液 苯扎氯铵 高效液相色谱法 含量测定 |
Determination of Benzalkonium Chloride in Olopatadine Hydrochloride Eye Drops by HPLC |
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| Authors: | HAN Wen-fang; HAN Bao-ping; ZHU Qi-deng; LI Xiao-qiang; XING Zhen-qi |
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| Affiliation: | HAN Wen-fang; HAN Bao-ping; ZHU Qi-deng; LI Xiao-qiang; XING Zhen-qi(Shandong Bausch & Lomb Freda Phar.Co.; Ltd.; Jinan 250101; China); |
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| Abstract: | Objective To establish an HPLC method for the determination of benzalkonium chloride in olopatadine hydrochloride eye drops.Methods A CN column was used with the mobile phase of acetonitrile-water-triethylamine(440:560:1).The phosphoric acid was used to adjust pH to 3.4.The flow rate was 1.0 mL/min.The detection wavelength was 210nm.Results The calibration curve was linear in the range of 0.05～0.15 mg/mL for benzalkonium chloride(r = 0.999 8).The average recovery was 99.7 %(n = 9,RSD = 0.69 %) for benzalkon... |
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| Keywords: | olopatadine hydrochloride eye drops benzalkonium chloride HPLC assay |
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