| 一种真菌DNA提取方法的改进 |
| |
| 引用本文： | 薛淑静,岳田利,关健,袁亚宏,高振鹏. 一种真菌DNA提取方法的改进[J]. 食品研究与开发, 2006, 27(4): 39-40 |
| |
| 作者姓名： | 薛淑静 岳田利 关健 袁亚宏 高振鹏 |
| |
| 作者单位： | 西北农林科技大学食品科学与工程学院,陕西,杨凌,712100 |
| |
| 基金项目： | 西部开发科技行动项目;科技部专项基金;教育部霍英东教育基金 |
| |
| 摘 要： | 根霉属真菌由于气生菌丝特别发达，菌丝容易产生褐色或黑色色素等，用以前文献报道的方法不易提取DNA或提取的DNA容易降解。本文采用改进的氯化苄法，得到的DNA质量好、产量高，可以直接用于后续的基因操作，此方法具有快速、简便、经济的优点，而且需要样品量少，可用于小剂量样品DNA的提取。
|
| 关 键 词： | 真菌 DNA提取 氯化苄 |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2005-11-23 |
AN IMPROVED METHOD FOR EXTRACTING FUNGAL DNA |
| |
| XUE Shu-jing,YUE Tian-li,GUAN Jian,YUAN Ya-hong,GAO Zhen-peng. AN IMPROVED METHOD FOR EXTRACTING FUNGAL DNA[J]. Food Research and Developent, 2006, 27(4): 39-40 |
| |
| Authors: | XUE Shu-jing YUE Tian-li GUAN Jian YUAN Ya-hong GAO Zhen-peng |
| |
| Affiliation: | College of Food Science and Engineering, Northwest Sic-Teeh University of Agriculture and Forestry , Yangling 712100,Shaanxi, China |
| |
| Abstract: | The mycelia of Rhizopus increases very rapidly. Aerial myeelium greyish brows ,and then colonies produces a diffusing yellow pigment in the medium. DNA isolation from the filamentous fungi is invalid by the classie methods or the loss of DNA can oecur during the isolation step. DNA from the filamentous fungi could be provided by the modified with benzyl chioride. And the quality of the extraction may satisfy the need of molecular analysis. The method is not only easy and rapid , but also effective to obtain DNA from small-scale DNA sample. |
| |
| Keywords: | fungi preparation of DNA benzyl chioride |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
