DETERMINATION OF β-GLUCAN IN WORT AND BEER BY ITS BINDING WITH CALCOFLUOR,USING A FLUORIMETRIC FLOW-INJECTION-ANALYSIS (FIA) METHOD |
| |
Authors: | José M. Sendra José V. Carbonell Maria J. Gosalbes Victoria Todo |
| |
Affiliation: | 1. Instituto de Agroquimica y Tecnología de Alimentos (CSIC). Jaime Roig 11, 46010 Valencia, Spain;2. Asociación de Investigación de Cerveza y Malta (INVESCEMA). Jaime Roig 11, 46010 Valencia, Spain |
| |
Abstract: | The FIA-fluorimetric methods for the determination of β-glucan in worts and beers have been investigated, modified and improved. Fluorescence is excited by using a mercury lamp and a 338 nm band-pass excitation filter, and detected using a 425 nm long-pass emission filter. It has been found that an increase of the ionic strength of the eluent, up to a maxium of 1% Sodium Chloride, increases the range of β-glucan molecular weights effectively bound by Calcofluor. The influence of the low molecular weight components of wort and beer: dextrins, aminoacids, etc, has been studied and found that their effect is negligible. The interference of wort and beer colour on the measurement of β-glucan has been quantified and several approaches are given to overcome it. Thirty-six European beers have been analyzed for β-glucan content. The possible detection of the addition of exogenous β-glucanases to wort and beer is discussed. |
| |
Keywords: | β-Glucan fluorescence analysis wort beer |
|
|