| 基于表面增强拉曼光谱的结肠癌组织免疫分析 |
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| 引用本文： | 席刚琴,陈燕坪,陈刚,郑雄伟,冯尚源,俞允,林筱茜,陈荣. 基于表面增强拉曼光谱的结肠癌组织免疫分析[J]. 中国激光, 2011, 0(9) |
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| 作者姓名： | 席刚琴 陈燕坪 陈刚 郑雄伟 冯尚源 俞允 林筱茜 陈荣 |
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| 作者单位： | 福建师范大学医学光电科学与技术教育部重点实验室福建省光子技术重点实验室;福建医科大学教学医院福建省肿瘤医院;福建省肿瘤医院病理科; |
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| 基金项目： | 国家自然科学基金(60778046,60910106016); 福建省科技项目(2008J0016,2009J01276); 卫生部科学研究基金-福建省卫生教育联合攻关计划(WKJ2008-2-046)资助课题 |
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| 摘 要： | 通过表面增强拉曼散射(SERS)标记抗体检测结肠癌组织切片上癌胚抗原(CEA)的表达,探讨SERS标记免疫检测技术用于临床分析结肠癌组织切片中蛋白质表达的可行性。采用种子生长法合成Ag壳Au核复合纳米粒子,将4-巯基苯甲酸(4-MBA)作为标记分子吸附于纳米粒子表面,制备出SERS探针;再将这种探针与CEA单克隆抗体相结合形成SERS免疫探针;最后通过SERS标记的抗体与结肠癌组织切片上相应的抗原发生特异性结合,对滴加探针的组织切片进行SERS检测和成像。结肠癌腺上皮出现很强的SERS信号,而除了少数非特异性吸附产生的信号之外,间质及正常上皮中几乎不出现SERS信号。通过SERS成像可以清晰地看到结肠癌腺上皮显示红色,表明结肠癌腺上皮高表达CEA,而间质及正常上皮几乎显示黑色和深蓝色,只有极个别点为红色,表明间质及正常上皮基本不表达CEA。研究表明,SERS标记抗体检测分析技术具有高灵敏度和高特异性,有望应用于结肠癌组织切片中蛋白质表达的分析,成为结肠癌辅助诊断的一种重要方法。
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| 关 键 词： | 光谱学 表面增强拉曼散射 免疫分析 纳米粒子 表面增强拉曼散射成像 结肠癌 组织切片 |
Surface-Enhanced Raman Scattering Spectroscopic Assay of Immunohistochemically Stained Human Colon Cancer Tissue |
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| Xi Gangqin Chen Yanping Chen Gang Zheng Xiongwei Feng ShangyuanYu Yun Lin Xiaoqian Chen RongKey Laboratory of Optoelectronic Science , Technology for Medicine,Ministry of Education,Fujian ProvincialKey Laboratory of Photonic Technology,Fujian Normal University,Fuzhou,Fujian ,ChinaTeaching Hospital Fujian Provincial Tumor Hospital,Fujian Medical University,Fujian ,ChinaPathology Department,Fujian Provincial Tumor Hospital,China. Surface-Enhanced Raman Scattering Spectroscopic Assay of Immunohistochemically Stained Human Colon Cancer Tissue[J]. Chinese Journal of Lasers, 2011, 0(9) |
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| Authors: | Xi Gangqin Chen Yanping Chen Gang Zheng Xiongwei Feng ShangyuanYu Yun Lin Xiaoqian Chen RongKey Laboratory of Optoelectronic Science Technology for Medicine Ministry of Education Fujian ProvincialKey Laboratory of Photonic Technology Fujian Normal University Fuzhou Fujian ChinaTeaching Hospital Fujian Provincial Tumor Hospital Fujian Medical University Fujian ChinaPathology Department Fujian Provincial Tumor Hospital China |
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| Affiliation: | Xi Gangqin1 Chen Yanping2 Chen Gang3 Zheng Xiongwei3 Feng Shangyuan1Yu Yun1 Lin Xiaoqian1 Chen Rong11Key Laboratory of Optoelectronic Science and Technology for Medicine,Ministry of Education,Fujian ProvincialKey Laboratory of Photonic Technology,Fujian Normal University,Fuzhou,Fujian 350007,China2Teaching Hospital Fujian Provincial Tumor Hospital,Fujian Medical University,Fujian 350014,China3Pathology Department,Fujian Provincial Tumor Hospital,China |
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| Abstract: | To explore the feasibility of surface-enhanced Raman scattering(SERS) labeled immunoassay technology for clinical analysis protein expression in colon cancer tissue sections,we develop 4-mercaptobenzoic acid(4-MBA) labeled Au/Ag core-shell nanoparticles(NPs) as assay platform for the detection of carcinoembryonic antigen(CEA) in colon cancer tissue samples.Firstly,Raman active molecule 4-MBA is adsorbed on Ag shell Au core bimetallic NPs,and then the SERS-tagged nanoprobes are modified with CEA monoclonal a... |
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| Keywords: | spectroscopy surface-enhanced Raman scattering immunoassay nanoparticles surface-enhanced Raman scattering imaging colon cancer tissue section |
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