| 枯草芽孢杆菌萌发后孢子响应甲醛胁迫过程的拉曼光谱研究 |
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| 引用本文： | 程琴,董蓉,黄大林,黄庶识,陈丽梅. 枯草芽孢杆菌萌发后孢子响应甲醛胁迫过程的拉曼光谱研究[J]. 中国激光, 2011, 0(10) |
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| 作者姓名： | 程琴 董蓉 黄大林 黄庶识 陈丽梅 |
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| 作者单位： | 广西科学院生物物理实验室;昆明理工大学生物工程技术研究中心;广西师范大学生命科学院;桂林医学院微生物与免疫学教研室; |
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| 基金项目： | 国家自然科学基金(30860010,30970263); 广西自然科学基金(桂科回0832006)资助课题 |
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| 摘 要： | 为了探究枯草芽孢杆菌萌发后孢子响应甲醛胁迫过程的生理反应及其耐受甲醛机理,利用激光光镊拉曼光谱(LTRS)系统研究不同浓度甲醛胁迫萌发后孢子2 h的响应过程。结果显示,营养细胞和萌发后孢子对甲醛都具有耐受能力,但萌发后孢子对不同浓度甲醛胁迫产生的生理响应效应不同;当萌发后孢子在不含甲醛的培养基中生长时,与生物大分子相应的特征峰强度增加先经历0~0.5 h的延缓期,接着1.5~2.0 h是一个快速上升的过程;当分别用0.4,0.8和1.0 mmol/L甲醛胁迫萌发后孢子时,相应特征峰强度都有一个先上升后下降的过程,峰强下降的时间拐点分别是1.5,1.0和0.5 h。光谱分析结果表明,0.4 mmol/L甲醛的胁迫会对细胞造成轻度伤害;0.8 mmol/L甲醛胁迫会抑制孢子DNA的复制,造成膜脂碳氢链断裂;1.0 mmol/L甲醛胁迫时,细胞在0.5 h内对甲醛产生了剧烈的胁迫响应,导致生物大分子含量在0.5~2.0 h内严重下降,细胞受到严重伤害。
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| 关 键 词： | 光谱学 拉曼光谱 单细胞分析 枯草芽孢杆菌 萌发后孢子 甲醛胁迫 |
Raman Spectrum of Germinated Spores of Bacillus Subtilis in Response to Formaldehyde Stress |
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| Cheng Qin, Dong Rong, Huang Dalin Huang Shushi, Chen Limei. Raman Spectrum of Germinated Spores of Bacillus Subtilis in Response to Formaldehyde Stress[J]. Chinese Journal of Lasers, 2011, 0(10) |
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| Authors: | Cheng Qin Dong Rong Huang Dalin Huang Shushi Chen Limei |
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| Affiliation: | Cheng Qin1,2 Dong Rong1,3 Huang Dalin4 Huang Shushi1,2 Chen Limei2 (1Laboratory of Biophysics,Guangxi Academy of Sciences,Nanning,Guangxi 530007,China 2Biotechnology Research Center,Kunming University of Science and Technology,Kunming,Yunnan 650224,China 3College of Life Science,Guangxi Normal University,Guilin,Guangxi 541004,China 4Department of Microbiology and Immunology,Guilin Medical University,China) |
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| Abstract: | In order to explore the physiological response and tolerance mechanism of Bacillus subtilis after exposure to formaldehyde,laser tweezers Raman spectroscopy(LTRS) is employed to perform the experimental process in which germinated spores(g.spores) are stressed with different concentrations of formaldehyde for 2 h.The results show that both vegetative cells and g.spores have tolerance to formaldehyde,but the growth and physiological effect of g.spores stressed by different concentrations of formaldehyde are ... |
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| Keywords: | spectroscopy Raman spectroscopy single cell analysis Bacillus subtilis germinated spores formaldehyde stress |
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