| 贵州黑糯米酒曲优良酵母菌分离筛选与鉴定 |
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| 引用本文： | 徐本刚,母应春,陈虹文,雷学昌,苏伟.贵州黑糯米酒曲优良酵母菌分离筛选与鉴定[J].中国酿造,2012,31(4):62-67. |
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| 作者姓名： | 徐本刚 母应春 陈虹文 雷学昌 苏伟 |
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| 作者单位： | 1. 贵州大学生命科学学院,贵州贵阳,550025
2. 贵州大学生命科学学院,贵州贵阳550025;贵州大学南区分析测试中心,贵州贵阳550025 |
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| 基金项目： | 贵州大学研究生创新基金(校研究农2011014);贵阳市科技局([2010)筑科农合同字第8-1号] |
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| 摘 要： | 从贵州黑糯米酒曲中分离出酵母菌312株。通过菌落形态菌体形态观察及生理生化实验将其初步鉴定至5个属。酵母属57株、假丝酵母属14株、复膜孢酵母属28株、毕赤酵母属212株、德巴利氏酵母属1株。经TTC法、杜氏管法、发酵法及玉米糖化醪法逐级筛选,最终筛选出1株产酒量高产香好的菌株,编号为Q3～J8。并提取菌株Q3-M8ITS序列进行分子生物学鉴定,Q3-J8为布拉酵母（Saccharomyces boulardii）。
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| 关 键 词： | 酒曲 酵母菌 分离 鉴定 |
Isolation and identification of fine saccharification molds from black glutinous rice wine starter from Guizhou |
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| XU Bengang
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MU Yingchun
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CHEN Hongwen
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LEI Xuechang
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SU Wei.Isolation and identification of fine saccharification molds from black glutinous rice wine starter from Guizhou[J].China Brewing,2012,31(4):62-67. |
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| Authors: | XU Bengang MU Yingchun CHEN Hongwen LEI Xuechang SU Wei |
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| Affiliation: | 1,2(1.College of Life Sciences,Guizhou University,Guiyang 550525,China;2.Southern Analysis and Test Center of Guizhou University,Guiyang 550025,China) |
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| Abstract: | A total number of 312 strains yeasts were obtained from black glutinous rice starter of Guizhou.By the methods of colonial morphology identification and physiology and biochemistry testing,the primary identification results showed that the starains were belonged to 5 categories.There were 57 Saccharomyces strains,14 Candida strains,Sacchromycopsis fibuligera strains,212 Pichiapastoris strains,1 Debaryomyces strain.Screened by the methods of TTC,Durham’s fermentation,zymotechnics,corn saccharification liquor,a strain numbered Q3 ～J8 which charactered by a high ability of ethanol producing and flavor.The ITS sequences was extracted for molecular biology identification,and the result showed that it was Saccharomyces boulardii. |
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| Keywords: | starter yeast isolation identification |
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