|
|||||
|
|
| 基因芯片技术鉴定食源性金黄色葡萄球菌 | |
| 引用本文： | 李玉锋,何洋,刘红露.基因芯片技术鉴定食源性金黄色葡萄球菌[J].食品研究与开发,2007,28(9):28-30. |
| 作者姓名： | 李玉锋 何洋 刘红露 |
| 作者单位： | 1. 西华大学生物工程学院,四川,成都,610039 2. 四川大学生命科学学院,四川,成都,610041 3. 四川省疾病控制预防中心,四川,成都,610031 |
| 基金项目： | 四川省应用基础研究计划;西华大学校科研和教改项目 |
| 摘 要： | 为了建立快速鉴定食派陛金黄色葡萄球菌的基因芯片技术，采用PCR方法扩增金黄色葡萄球菌16SrRNA基因的DNA片段．序列进行BLAST比较并通过软件设计其特异性探针，采用基因芯片杂交技术鉴定食源性金黄色葡萄球菌样品。结果：对所有样品进行基因芯片杂交技术处理并扫描观察，金黄色葡萄球菌的杂交结果呈阳性，其检测结果与传统方法鉴定结果一致。结论：应用基因芯片鉴定技术可快速、准确地鉴定食源性金黄色葡萄球菌，值得推广应用。 |
| 关 键 词： | 金黄色葡萄球菌 基因芯片 鉴定 |
| 修稿时间： | 2007-01-10 |
IDENTIFICATION OF FOOD-BORNE STAPHYLOCOCCUS A UREUS BY THE MICROARRAY TECHNIQUE |
|
| LI Yu-Feng,HE Yang,LIU Hong-Lu.IDENTIFICATION OF FOOD-BORNE STAPHYLOCOCCUS A UREUS BY THE MICROARRAY TECHNIQUE[J].Food Research and Developent,2007,28(9):28-30. | |
| Authors: | LI Yu-Feng HE Yang LIU Hong-Lu |
| Affiliation: | 1.College of Bioengineering, Xihua University, Chengdu 610039, Sichuan, China;2 .College of Life Science, Sichuan University, Chengdu 610041, Sichuan, China; 3.The Sichuan Center of Disease Control, Chengdu 610031, Sichuan, China |
| Abstract: | The goal of this study was to establish a method for identifying rapidly food-borne Staphylococcus aureus by microarray technology. The target gene of 16SrRNA form S. aureus was amplified by PCR and the sequence was compared for BLAST in GenBank. The Oligonucleotide hybridization probe was designed to identify sample. Results: The specimens were detected by microarray hybridization. The result of microarray hybridisation for food-borne Staphylococcus aureus showed that it is positive. Conclusion: The food-borne Staphylococcus aureus was identified rapidly and accurately with microarray hybridization technique that it is worth propagation. |
| Keywords: | Stap hylococc us aure us microarray identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |