| 微波辅助萃取液相色谱快速检测烟草中的多酚类化合物 |
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| 引用本文： | 赵瑞峰,胡静,李峰.微波辅助萃取液相色谱快速检测烟草中的多酚类化合物[J].现代食品科技,2011,27(8):1036-1039. |
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| 作者姓名： | 赵瑞峰 胡静 李峰 |
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| 作者单位： | 广东中烟工业有限责任公司技术中心,广东广州,510145 |
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| 摘 要： | 建立了卷烟烟丝中三个主要多酚化合物(绿原酸、莨菪亭、芸香苷)的超高效液相色谱(UPLC)的快速检测方法.采用微波辅助萃取法(microwave-assisted extraction MAE)分别进行单因素试验和正交试验,确定卷烟烟丝中三个主要多酚类化合物的最佳提取条件:60%(V/V)的甲醇水溶液为提取剂、萃取液温度...
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| 关 键 词： | 烟丝 微波辅助萃取 绿原酸 莨菪亭 芸香苷 |
Fast Determination of Phenolic Compounds in Tobacco by Microwave-assisted Extraction-ultrahigh Performance Liquid Chromatography |
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| ZHAO Rui-feng,HU Jing,LI Feng.Fast Determination of Phenolic Compounds in Tobacco by Microwave-assisted Extraction-ultrahigh Performance Liquid Chromatography[J].Modern Food Science & Technology,2011,27(8):1036-1039. |
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| Authors: | ZHAO Rui-feng HU Jing LI Feng |
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| Affiliation: | (Technology Centre of China Tobacco Guangdong Industrial Co.,Ltd,Guangzhou 510145,China) |
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| Abstract: | A fast microwave-assisted extraction-ultrahigh performance liquid chromatography(MAE-UPLC) method was developed for the simultaneous determination of chlorogenic acid,scopoletin and rutin in cut tobacco.The extraction conditions of polyphenols were determined by single-factor and the orthogonal experiment.The optimal conditions were determined as follows: methanol concentration 60%(V/V),extraction solvent temperature 94 ℃,microwave treatment time 1min and solid to liquid ratio 1:20(g/mL),under which the recovery rate of polyphenols in cut tobacco were 96.5%-105.6% and the relative standard deviation was lower than 5%. |
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| Keywords: | cut tobacco scopoletin chlorogenic acid rutin microwave-assisted extraction |
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