| 镍离子增强辣根过氧化物酶法合成联苯研究 |
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| 引用本文： | 肖保林,洪军,王玮,黄琨,杨卫云,赵莹雪,穆萨维姆瓦赫迪. 镍离子增强辣根过氧化物酶法合成联苯研究[J]. 广州化工, 2011, 39(12): 49-51,56 |
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| 作者姓名： | 肖保林 洪军 王玮 黄琨 杨卫云 赵莹雪 穆萨维姆瓦赫迪 |
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| 作者单位： | 1. 河南大学生命科学学院,河南开封,475000
2. 德黑兰大学生物化学与生物物理研究所，伊朗，德黑兰 |
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| 基金项目： | 河南大学特聘教授科研启动基金和伊朗国家科学基金资助。 |
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| 摘 要： | 通过2 mM Ni2+孵化辣根过氧化物酶，可制备活化辣根过氧化物酶(Ni2-HRP)，实验验证了Ni2-HRP的活性和稳定性，并对Ni2-HRP催化2-二叔丁基苯酚反应的生成物进行了CC/MS，HPLC和核磁共振鉴定，确定为2，4，2′,4′-四羟基-5，5′-二叔丁基联苯，是一种几乎无毒的化合物，可作为制药前体的使用...
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| 关 键 词： | 镍离子 辣根过氧化物酶 联苯 |
Biochemical Synthesis of Biphenyl from 2 - tert - butylphenol by Horseradish Peroxidase Activated with Ni2+ |
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| XIAO Bao-lin,HONG Jun,WANG Wei,HUANG Kun,YANG Wei-yun,ZHAO Ying-xue,MOOSA VI-MOVAHEDI Ali-Akbar. Biochemical Synthesis of Biphenyl from 2 - tert - butylphenol by Horseradish Peroxidase Activated with Ni2+[J]. GuangZhou Chemical Industry and Technology, 2011, 39(12): 49-51,56 |
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| Authors: | XIAO Bao-lin HONG Jun WANG Wei HUANG Kun YANG Wei-yun ZHAO Ying-xue MOOSA VI-MOVAHEDI Ali-Akbar |
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| Affiliation: | XIAO Bao-lin1,HONG Jun1,WANG Wei1,HUANG Kun1,YANG Wei-yun1, ZHAO Ying-xue1,MOOSAVI-MOVAHEDI Ali-Akbar2(1 College of Life Science,Henan University,Henan Kaifeng 475000,China;2 Institute of Biochemistry and Biophysics,University of Tehran,Tehran Iran) |
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| Abstract: | Activated horseradish peroxidase(Ni2+-HRP) was prepared via incubation with Ni2+(2 mM).The experiment results showed the activity and stability of Ni2+-HRP,and the purified products were identified by NMR,GC-Mass and HPLC.The product was "2,4,2’,4’-tetrahydroxy-5,5’-di-tert-butyl-biphenyl" as a mostly nontoxic,and can be usable as pharmaceutical precursors. |
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| Keywords: | Ni2+ activated horseradish peroxidase biphenyl |
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