| 产溶菌酶白色链霉菌的筛选及鉴定 |
| |
| 引用本文： | 杨辉,闫守泉,赖祯冬,林祖端,梁海秋,朱萍. 产溶菌酶白色链霉菌的筛选及鉴定[J]. 现代食品科技, 2011, 27(8): 881-884 |
| |
| 作者姓名： | 杨辉 闫守泉 赖祯冬 林祖端 梁海秋 朱萍 |
| |
| 作者单位： | 广西大学生命科学与技术学院,广西南宁,530005 |
| |
| 基金项目： | 广西壮族自治区自然科学基金项目 |
| |
| 摘 要： | 从广西各地土壤中取样,利用添加金黄色葡萄球菌的LB培养基初筛,然后用含金黄色葡萄球菌的营养盐平板复筛,所得突变株经摇瓶发酵后测定其用蛋白酶K处理前后的粗酶液活力变化,筛选到一株溶菌酶产生菌RJ-36b.通过菌株形态学和16S rDNA保守序列分析,鉴定其为白色链霉菌.对其发酵条件的初步研究表明,在32℃、200 r/m...
|
| 关 键 词： | 溶菌酶 白色链霉菌 鉴定 酶活力 |
Screening and Identification of a Lysozyme-producing Streptomyces albus Strain |
| |
| YANG Hui,YAN Shou-quan,LAI Zhen-dong,LIN Zu-duan,LIANG Hai-qiu,ZHU Ping. Screening and Identification of a Lysozyme-producing Streptomyces albus Strain[J]. Modern Food Science & Technology, 2011, 27(8): 881-884 |
| |
| Authors: | YANG Hui YAN Shou-quan LAI Zhen-dong LIN Zu-duan LIANG Hai-qiu ZHU Ping |
| |
| Affiliation: | (College of Life Science and Technology of Guangxi University,Nanning 530005,China) |
| |
| Abstract: | A lysozyme-producing strain was obtained from soil using the LB plate and PBS plate both contained Staphylococcus aureus,followed by detecting activity changes after treatment with protease K.Through analysis of physiological properties and the sequence of 16S rDNA,it was finally classified into Streptomyces albus sp.RJ-36 b.Results suggested that its enzyme-producing process was closely relevant to its growth.The maximal activity(108 U/mL) was achieved in shake flask at 32 ℃,200 r/min for 60 h. |
| |
| Keywords: | lysozyme Streptomyces albus identification enzyme activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
