不同培养条件对冬虫夏草菌丝体甘露醇的影响

目的:探讨不同培养条件对冬虫夏草菌丝重量和甘露醇含量的影响。方法:甘露醇的测定采用比色方法,菌丝干燥后直接称重。结果对于菌丝的产量,摇菌时间不应超过16d,最佳氮源为硝酸铵。对培养液和菌丝中甘露醇的总量,摇菌时间不应超过8d,最佳氮源为氯化铵。结论:氮源和摇菌时间不同,对冬虫夏草菌的菌丝重量和甘露醇含量有影响。

Effects on Determining Mannitol Content of Myceliun of Cordyceps Sinensis by Different Culture Condition

Object: To study the effects of mannitol content and mycelium weight by different culture condition in mycelium of cordyceps sinensis. Method: Colorimetric method was used to determine mannitol content. Mycelium weight was weighted after drying. Result: The mycelium weight was maximum at 16 days of shaking with ammonium nitrate as the optimum source of nitrogen. To obtain a high mannitol content in culture and mycelium, it was necessary to have 8 days of shaking and the optimum source of nitrogen was ammonium ohloride. Conclusion: The mannitol content and mycelium weight were affected by shaking time and source of nitrogen.