Magnetic beads based immunoaffinity capillary electrophoresis of total serum IgE with laser-induced fluorescence detection |
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Authors: | Chen Hong-Xu Busnel Jean-Marc Peltre Gabriel Zhang Xin-Xiang Girault Hubert H |
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Affiliation: | Beijing National Laboratory for Molecular Sciences, College of Chemistry, Peking University, Beijing 100871, China. |
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Abstract: | A magnetic beads based immunoaffinity capillary electrophoresis method for total Immunoglobulin E quantification in serum has been developed. The method combines speed, automation ability, and minimal sample consumption. Only 1 microL of serum is required while the whole immunoaffinity capillary electrophoresis method is performed in less than 50 min. The concomitant use of online immunocapture, transient isotachophoresis, and laser-induced fluorescence detection provides a sensitivity in the low picomolar range and a highly linear fluorescence response over 4 orders of magnitude (IgE concentration ranging from 2.4 to 2400 ng/mL). After validation with a reference material, the method has been successfully applied to the quantification of total IgEs in patient sera. The results compared well with classical ImmunoCap data. |
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