CMOS chip as luminescent sensor for biochemical reactions |
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Authors: | Ude Lu Hu BC-P Yu-Chuan Shih Yuh-Shyong Yang Chung-Yu Wu Chiun-Jye Yuan Ming-Dou Ker Tung-Kung Wu Yaw-Kuen Li You-Zung Hsieh Wensyang Hsu Chin-Teng Lin |
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Affiliation: | Dept. of Biol. Sci. & Technol., Nat. Chiao Tung Univ., Hsinchu, Taiwan; |
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Abstract: | We describe a novel biochemical sensing method and its potential new biosensing applications. A light-sensitive complementary metal oxide semiconductor (CMOS) chip prepared through a standard 0.5-/spl mu/m CMOS process was developed for measuring biochemical reactions. A light producing enzymatic reaction catalyzed by horseradish peroxidase (HRP) was designed as a platform reaction to determine the concentration of hydrogen peroxide (H/sub 2/O/sub 2/) by the CMOS chip with a standard semiconductor parameter analyzer (HP4145). The kinetics of enzymatic reaction were determined and compared with a standard and sophisticated fluorometer (Hitachi F-4500) in a biochemical laboratory. Similar results were obtained by both instruments. Using glucose oxidase as an example, we further demonstrated that the HRP platform can be used to determine other H/sub 2/O/sub 2/ producing reactions with the CMOS system. The result points to an important application of the CMOS chip in biological measurements and in diagnosis of various health factors. |
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