聚驱后油藏产多糖微生物多样性与功能分析

通过16SrDNA克隆建库方法对大庆北二西油藏微生物调剖过程中和调剖后4个不同油井(B2-D4-P45井、B2-4-P47井、B2-4-P49井和B2-5-51井)3个不同时间段的样品经选择富集后的产多糖微生物群落进行研究。结果表明,主要的产多糖菌为爱媛类芽孢杆菌(Paenibacillus ehimensis)和爱媛芽孢杆菌(Bacillus ehimensis),经分离纯化得到一株纯菌DT-1,与爱媛类芽孢杆菌的16SrDNA序列同源性达到99%。调剖过程中主要激活了3类产多糖菌,调剖1年后微生物多样性明显增加,产生物表活剂菌和降解石油烃菌都被共同激活。

The Diversities and Functions of Polysaccharide Microbia Produced in the Reservoirs after Ploymer Flooding

16S rDNA clone library technique was used to analyze relative abundances of compositions of ploysaccharide bacterial profile control in the enrichment cultures of produced water in 3 different intervals from 4 different wells(such as Well B2-D4-P45,Well B2-4-P47,Well B2-4-P49 and B2-5-51) in the west of Region 2 in the north of Daqing Oilfield after polymer flooding.Results show that the dominant ploysaccharide bacteria are Paenibacillus ehimensis.A strain of DT-1 is isolated,phylogenetic analysis based on 16S rDNA gene sequence indicates that strain DT-1 is affiliated with Paenibacillus ehimensis with a similarity of 99%.During the process of the microbial plugging,three types of ploysaccharide bacteria are stimulated.One year after profile control,microbial diversities increase significantly,and biosurfactant-producing bacteria and petroleum hydrocarbon-degrading bacteria are co-activated.