拟目乌贼生殖腺碱提多糖的抗氧化及吸湿保湿特性

以拟目乌贼生殖腺为材料，采用碱法提取多糖，并对其抗氧化活性和吸湿保湿性进行研究。结果表明：拟目乌贼生殖腺碱提多糖最佳工艺为NaOH溶液浓度0.3 mol/L、料液比1∶40（g/mL）、提取时间3 h、提取温度80 ℃，此条件下多糖得率为7.697%。拟目乌贼生殖腺碱提多糖具有良好的抗氧化活性，对羟自由基和1,1-二苯基-2-三硝基苯肼自由基具有较好的清除效果，清除率与多糖质量浓度呈正相关。拟目乌贼生殖腺碱提多糖的吸湿性能高于壳聚糖、聚乙二醇6000等常规保湿剂，保湿性能和壳聚糖、聚乙二醇6000、丙三醇接近；吸湿保湿能力均高于翡翠贻贝多糖、近江牡蛎多糖等海洋动物多糖。综上表明，拟目乌贼生殖腺碱提多糖作为天然的抗氧化剂和保湿剂具有一定的应用前景。

Antioxidant Activity,Moisture Absorption and Retention Capacities of Alkali-Soluble Polysaccharides Extracted from Gonad of Sepia lycidas

The extraction of alkali-soluble polysaccharides from the gonad of Sepia lycidas was investigated, and the
antioxidant activity, moisture absorption and retention capacities of the extracted polysaccharides were evaluated. The
optimal extraction conditions were determined as follows: NaOH concentration, 0.3 mol/L; solid/liquid ratio, 1:40; extraction
time, 3 h; and extraction temperature, 80 ℃, respectively. Under these conditions, the extraction yield of polysaccharides
was 7.697%. Antioxidant assays showed that the polysaccharides possessed good radical scavenging activity against
hydroxyl and DPPH radicals in a marked dose-dependent manner. Moisture absorption capacity of Sepia lycidas gonad
polysaccharides was better than that of chitosan, polyethylene glycol 6000 and other conventional humectants, and moisture
retention capacity was close to that of chitosan, polyethylene glycol 6000 and glycerol. The moisture absorption and
retention capacity of Sepia lycidas gonad polysaccharides were better than those of the polysaccharides from Perna viridis,
Crassostrea rivularis and other marine animals. These results clearly established the possibility that Sepia lycidas gonad
polysaccharides could be effectively employed as a natural antioxidant and moisturizer.