| 富硒发芽糙米蛋白的抗氧化活性 |
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| 引用本文： | 胡玲玲,李春阳,曾晓雄,吴建苏.富硒发芽糙米蛋白的抗氧化活性[J].食品科学,2016,37(1):99-103. |
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| 作者姓名： | 胡玲玲 李春阳 曾晓雄 吴建苏 |
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| 作者单位： | 1.江苏省农业科学院农产品加工研究所，江苏 南京 210014；2.南京农业大学食品科技学院，江苏 南京 210095；
3.南京远望富硒农产品有限责任公司，江苏 南京 211500 |
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| 摘 要： | 采用碱提酸沉法提取了糙米和富硒发芽糙米中的蛋白，对其抗氧化活性进行分析测定，并与对照品芦丁和VC进行了比较。结果表明：富硒发芽糙米蛋白的总抗氧化能力（total antioxidant capacity，T-AOC）、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除率、超氧阴离子自由基（O2－·）清除率和还原力低于芦丁和VC，氧化自由基吸收能力（oxygen radical absorbance capacity，ORAC）显著低于芦丁（P＜0.05），但是显著高于糙米蛋白（P＜0.05）；富硒发芽糙米蛋白的羟自由基（·OH）清除能力与芦丁和VC相当，但显著高于糙米蛋白（P＜0.05）。富硒发芽后的糙米蛋白抗氧化能力显著提高。
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| 关 键 词： | 富硒发芽糙米 蛋白 抗氧化 清除自由基 |
Antioxidant Activity of Selenium-Enriched Germinated Brown Rice Protein |
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| HU Lingling,LI Chunyang,ZENG Xiaoxiong,WU Jiansu.Antioxidant Activity of Selenium-Enriched Germinated Brown Rice Protein[J].Food Science,2016,37(1):99-103. |
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| Authors: | HU Lingling LI Chunyang ZENG Xiaoxiong WU Jiansu |
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| Affiliation: | 1. Institute of Agro-Food Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
3. Jiangsu Yuanwang Selenium-rich Agricultural Produce Co. Ltd., Nanjing 211500, China |
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| Abstract: | In this study, selenium-enriched germinated brown rice protein was extracted using alkali extraction and acid
precipitation, and its antioxidant activity was compared with that of the reference substances of rutin and VC. The results
demonstrated that total antioxidant capacity (T-AOC), DPPH free radical scavenging activity, superoxide anion free radical
scavenging activity, and reducing power of the protein were lower than those of rutin and VC, and its oxygen radical absorbance
capacity (ORAC) was significantly lower than that of rutin (P < 0.05), but significantly higher than that of the protein extracted
from the ungerminated ordinary brown rice protein (P < 0.05). Hydroxyl radical scavenging activity of Se-enriched germinated
brown rice protein was similar to that of rutin, but significantly higher than that of ordinary brown rice protein (P < 0.05).
Therefore, antioxidant activity of the protein from se-enriched germinated brown rice was significantly improved. |
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| Keywords: | Se-enriched germinated brown rice protein antioxidant activity free radical scavenging |
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