用于农药残留现场快速检测的微流控芯片研制

研制一种用于农药残留现场快速检测的微流控芯片。设计制作的一次性高聚物微流控芯片集成进样、酶抑制反应、显色反应及检测单元，结合自制的手持式光度分析检测装置，可以实现对有机磷、氨基甲酸酯类农药的现场、低成本、快速、准确的检测。结果表明：通过在芯片内部固定存储生化试剂，只需一次进样，7 min内即可实现对克百威和乐果的快速检测，最低检出限分别为0.02、0.6 mg/L，对克百威加标回收率为95.0%～103.3%，制作的芯片在1 个月内的稳定性较好。使用微流控农药残留快速检测芯片系统，有望实现全自动检测流程，特别适合于基层非专业人员开展现场、快速、高通量的农药残留筛查。

Fabrication and Analytical Application of Microfludic Chip for Rapid On-Site Detection of Pesticide Residues

A novel disposable microfludic chip was developed for rapid detection of pesticide residues in agricultural
products, which integrated sampling, enzyme inhibition reaction, color development and detection chamber. By coupling
with a laboratory-prepared colorimetric detection device, the chip could provide a simple, easy to use, low-cost and sensitive
approach for rapid detection of organophosphate and carbamate pesticides on site. Due to pre-storage of biochemical
reagents in the chip, the detection could be achieved within 7 min by just one injection. The limits of detection (LOD) of
the method were 0.02 mg/L for carbofuran and 0.6 mg/L for dimethoate, the recoveries of carbofuran from spiked samples
were in the range of 95.0% to 103.3%. Furthermore, this microfludic chip had a long storage life of up to 30 days. The
microfluidic chip coupled with laboratory-prepared detection device is expected to achieve an automatic detection process,
and is particularly suitable for on-site, rapid, and high-throughput screening of pesticide residues by non-professionals.