添加维生素E的雨生红球藻亚急性毒性评价及增强免疫作用研究

研究了添加维生素E的雨生红球藻(HPE)对大鼠亚急性毒性和增强小鼠免疫功能作用。以1670、835、418mg/kg bw (分别相当于人体推荐用量100、50、25倍)三个剂量的HPE连续给大鼠灌胃30 d进行亚急性毒性试验；以334、167、84 mg/kg bw三个剂量连续给小鼠灌胃30 d进行免疫功能试验,结果显示,亚急性毒性试验期间动物的生长发育良好,各剂量组的动物体重、增重量、进食量、食物利用率、血常规指标、血生化指标、脏器重量及脏器/体重比值与对照组比较,均无显著性差异(P>0.05)；大体解剖观察和组织病理学检查未见与添加维生素E的雨生红球藻有关的异常改变。在受试剂量范围内未见添加维生素E的雨生红球藻对大鼠各项观察指标产生毒副作用。与对照组相比,中、高剂量组显著促进小鼠的脾淋巴细胞增殖转化能力,提高小鼠的血清溶血素水平；高剂量组显著提升了迟发型变态反应能力和小鼠碳廓清吞噬指数,明显增强抗体生成细胞能力。表明添加维生素E的雨生红球藻安全性高,具有增强免疫功能。

Study on subacute toxicity evaluation and immune enhancement of Haematococcus pluvialis added with vitamin E

Objective To explore the effects of Haematococcus pluvialis added with vitamin E (HPE) on subacute toxicity evaluation in rats and immune function in mice. Methods In the subacute toxicity test, the rats were administered at dosages of 1670, 835, 418, 0 mg/(kg·bw) by gavage once a day for 30 consecutive days. In immune function tests, the mice were administered at dosages of 334, 167, 84, 0 mg/(kg·bw) by gavage once a day for 30 consecutive days. Results In the subacute toxicity test of rats, compared with the control group, three groups HPE did not show toxicity. The animals grew well, and there was no significant difference between the control group and each dose group in terms of animal weight, weight gain, food intake, food utilization rate, blood routine indicators, blood biochemical indicators, organ weight; no abnormal changes related to HPE were found in gross anatomical observation and histopathological examination. In immune function tests, compared with the control group, the medium and high dose groups significantly promoted the proliferation and transformation of splenic lymphocytes, and increased the level of serum hemolysin in mice; the high dose group significantly enhanced the delayed type hypersensitivity and the carbon clearance phagocytic index of mice, and significantly enhanced the antibody producing cell capacity. Conclusion It is confirmed that HPE treatments significantly improve cellular and humoral immune functions without subacute toxicity.