Pilot-scale production of bacterial cellulose by a spherical type bubble column bioreactor using saccharified food wastes |
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Authors: | Hyo-Jeong Song Hongxian Li Jin-Ho Seo Myong-Jun Kim Seong-Jun Kim |
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Affiliation: | (1) Department of Urban Engineering, Chonnam National University, Daejeon, 305-764, Korea;(2) Department of Civil, Earth and Environmental Engineering, Chonnam National University, Gwangju, 500-757, Korea |
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Abstract: | Bacterial cellulose (BC) was produced by Acetobacter xylinum KJ1 in a modified airlift-type bubble column bioreactor, which had a low shear stress and high oxygen transfer rate (k
L
a). Saccharified food wastes (SFW) were used as the BC production medium due to its low cost. An aeration rate of 1.2 vvm
(6 L/min) was tentatively determined as the optimal aeration condition in a 10 L spherical type bubble column bioreactor,
by analysis of the oxygen transfer coefficient. When 0.4% agar was added, the BC production reached 5.8 g/L, compared with
5.0 g/L in the culture without the addition of agar. The BC productivity was improved by 10% in the addition of 0.4% agar
into the SFW medium. Then, by conversion of a linear velocity of 0.93 cm/sec, from the relationship between the linear velocity
and oxygen transfer rate, 1.0 vvm (30 L/min) was determined as an optimal aeration condition in a 50 L spherical type bubble
column reactor. Using SFW medium, with the addition of 0.4% agar, and air supplied of 1.0 vvm, 5.6 g/L BC was produced in
the 50 L spherical type bubble column bioreactor after 3 days of cultivation, which was similar to that produced in the 10
L bioreactor. In conclusion, the addition of agar, a viscous polysaccharide, into SFW medium is effective for the production
of BC, and this scale-up method is very useful for the mass production in a 50 L spherical type bubble column bioreactor by
decreasing the shear stress and increasing the k
L
a. |
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Keywords: | Scale-up Bacterial Cellulose Saccharified Food Wastes Spherical Type Bubble Column Bioreactor |
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