Immobilization and the conformational study of phospholipid and phospholipid-protein vesicles

The present work demonstrates the immobilization of phospholipids and phospholipids-protein vesicles on solid substrate by simple drop-cast technique. We have also studied the structural and conformational transition of protein (OVA) molecules when they are loaded in immobilized vesicles of DPPC. High-resolution FE-SEM imaging is used to study the structural aspects of the immobilized vesicles of DPPC and OVA loaded DPPC. FTIR analyses of amide bands are being used to inspect the extent of the conformational transitions of β-sheet to α-helix of OVA in immobilized DPPC vesicles. Immobilized OVA-DPPC vesicles provide the structure of individual OVA molecule attached with DPPC, without aggregation amongst them.