Glow discharge plasma-induced immobilization of heparin and insulin on polyethylene terephthalate film surfaces enhances anti-thrombogenic properties |
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Authors: | K Navaneetha Pandiyaraj V Selvarajan Young Ha Rhee Hyoung Woo Kim S Ismat Shah |
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Affiliation: | 1. Plasma Physics Laboratory, Department of Physics, Bharathiar University, Coimbatore-641 046, India;2. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305-764, South Korea;3. Department of Physics and Astronomy, Department of Materials Science and Engineering, University of Delaware, 208 Dupont Hall, Newark, United States |
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Abstract: | Polyethylene terephthalate (PET) films were treated with DC glow discharge plasma followed by graft copolymerization with acrylic acid (AA) and polyethylene glycol (PEG). The obtained PET–PEG was coupled to heparin or insulin molecules. The surfaces were then characterized by contact angle measurements, atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The surface energies of the modified PET films were estimated using contact angle measurements, and the changes in crystallinity of the plasma-modified PET film surfaces were investigated by X-ray diffraction (XRD) analysis. The blood compatibilities of the surface-modified PETs were examined by in vitro thrombus formation, whole blood clotting time, platelet contact and protein adsorption experiments. The results revealed that the contact angle value decreased and that the interfacial tension between the modified PET films and blood protein was drastically diminished compared to unmodified PET film. The XPS results showed that the PET–AA surface containing carboxylic acid and the immobilized PET surface containing both carboxylic acid and amino groups exhibited a hydrophilic character, and AFM results showed marked morphological changes after grafting of AA, PEG and biomolecule immobilization. Heparin and insulin-coupled PET surfaces exhibited much less platelet adhesion and protein adsorption than the other surface-modified PET film surfaces. |
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