Composite gel-filled giant vesicles: Membrane homogeneity and mechanical properties |
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Authors: | Clément C. Campillo André P. Schroder Carlos M. Marques Brigitte Pépin-Donat |
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Affiliation: | 1. Structure et Propriétés d’Architectures Moléculaires, UMR 5819(CEA-CNRS-UJF)/INAC/CEA-Grenoble; 17, rue des Martyrs, 38054 Grenoble Cedex 9, France;2. Institut Charles Sadron, Univ. Louis Pasteur, CNRS - UPR 22; 23, rue du Loess, BP 84047, 67034 Strasbourg Cedex 2, France |
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Abstract: | We further investigate the properties of composite Poly(NIPAM) (poly(N-isopropylacrylamide)) gel-filled giant vesicles, focusing here on i) the homogeneity of the membrane, ii) its coupling to the inner gel under strong suction pressures, and iii) the relation between the final elastic modulus of the vesicles and the amount of crosslinker in the pre-gel medium. We show that whereas the photo-polymerization process induces a decrease of the membrane homogeneity at the micrometer size range, the membrane still remains strongly coupled to the internal gel network. The vesicles studied here display average moduli in the range [0.5–25] kPa, confirming their potential as biomimetic mechanical systems. |
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