Isolation of eriocitrin (eriodictyol 7‐O‐rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit (Citrus lumia) and its distribution in Citrus species |
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| Authors: | Yoichi Nogata Hideaki Ohta Toshinao Ishii Keizo Sekiya |
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| Affiliation: | 1. National Agricultural Research Center for Western Region, Fukuyama, Hiroshima 721‐8514, Japan;2. Department of Nutritional Science, Nakamura Gakuen University, Befu, Fukuoka 814‐0198, Japan |
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| Abstract: | An inhibitory compound acting against rat platelet 12‐lipoxygenase was isolated from the peel of Lumie fruit (Citrus lumia) by activity‐guided separation. It was identified as eriocitrin (eriodictyol 7‐O‐rutinoside) by spectroscopic analyses. Eriocitrin inhibited 5‐lipoxygenase (IC5029.1 µmol L?1) from rat peritoneal polymorphonuclear leukocytes in addition to 12‐lipoxygenase (IC5022.3 µmol L?1). Its aglycone, eriodictyol (5,7,3′, 4′‐tetrahydroxyflavanone), was a much more potent inhibitor of both 12‐lipoxygenase (IC500.07 µmol L?1) and 5‐lipoxygenase (IC500.20 µmol L?1). It also inhibited the production of leukotriene B4 in intact peritoneal polymorphonuclear leukocytes stimulated with calcium ionophore A23187 (IC5012.7 µmol L?1). The distribution of eriocitrin in 39 citrus fruits was investigated by high‐performance liquid chromatography analysis. Lumie, eureka lemon (Citrus limon), Sambokan (Citrus sulcata), Sudachi (Citrus sudachi) and Koji (Citrus leiocarpa) fruits were found to contain high levels of eriocitrin in both peel and juice vesicles. Copyright © 2006 Society of Chemical Industry |
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| Keywords: | eriocitrin eriodictyol arachidonate lipoxygenase inhibitor Lumie lemon Sudachi Sambokan Koji |
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