| 血红素提取纯化新技术研究 |
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| 引用本文： | 宋照军,刘玺,赵良,张培旗. 血红素提取纯化新技术研究[J]. 现代食品科技, 2004, 20(1): 6-8 |
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| 作者姓名： | 宋照军 刘玺 赵良 张培旗 |
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| 作者单位： | 河南职业技术师范学院,新乡,453003;中国海洋大学,青岛,266003 |
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| 摘 要： | 本文对猪血红细胞中血红素的提取工艺进行了研究,通过正交试验确定了提取纯化血红素的最优工艺条件,即:溶血时,添加去离子水1倍,氯仿0.15倍;分离血红素时,加丙酮4倍、同时调节PH值3;结晶血红素时加NaAc1.0%,调节PH值为5。根据本工艺可从1000ml猪血红细胞中提取2500mg、纯度达90%的血红素。
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| 关 键 词： | 猪血 提取 纯化 血红素 技术 研究 |
| 文章编号： | 1007-2764(2004)01-0003-06 |
| 修稿时间： | 2003-09-29 |
Study on New Technology of Extracting and Sublimating Heme |
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| Song Zhaojun,Zhao Liang,Liu Xi. Study on New Technology of Extracting and Sublimating Heme[J]. Modern Food Science & Technology, 2004, 20(1): 6-8 |
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| Authors: | Song Zhaojun Zhao Liang Liu Xi |
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| Abstract: | This paper researches the technological procedure of heme extracting and sublimating from pig red cells. Through perpendicular testing, we found the excellent procedure condition. during hemolysis, add water 3 times, chloroform 0.25 times, and regulate ph3.0. During extracting, add NaAc 1.0%, regulate ph5.0.By this procedure, we obtained heme 2500mg weight and 90% purity from 1000 ml pig blood. |
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| Keywords: | Pig blood Extraction Sublimate Heme Techniques Study |
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