A simple and rapid method for histamine determination in fermented sausages by mediated chronopotentiometry |
| |
| Authors: | Zorica S. Stojanović Jaroslava V. Švarc-Gajić |
| |
| Affiliation: | 1. Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 61265 Brno, Czech Republic;2. Masaryk Memorial Cancer Institute, ?lutý kopec 7, 65653 Brno, Czech Republic;1. University of Novi Sad, Faculty of Technology Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia;2. Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic;3. Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 612 00 Brno, Czech Republic;4. CEITEC — Central European Institute of Technology, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic;1. Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, Universiteit Antwerpen, Groenenborgerlaan 171, 2020, Antwerpen, Belgium;2. Physical Chemistry and Soft Matter, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands;1. AIT Austrian Institute of Technology, Center for Health & Bioresources, Konrad Lorenz Strasse 24, 3430 Tulln, Austria;2. Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France |
| |
| Abstract: | In order to provide the basis for the development of an inexpensive, sensitive, and reliable method for histamine determination in foods, the electrochemical behavior of histamine at planar gold disc electrode was investigated by chronopotentiometry. In solutions with sufficient chloride content dual oxidative mechanism of histamine was observed and included catalytic effect of electrogenerated chlorine. Well defined signal observed at +1.15 V in 0.0025 mol/l hydrochloric acid demonstrated sensitive changes with histamine content and was attributed to histamine. Electrogenerated chlorine facilitated charge transfer between histamine and the gold electrode resulting in enhancement of sensitivity. The effects of the most important experimental parameters of the chlorine-mediated chronopotentiometric analysis, such as the type and concentration of supporting electrolyte, initial potential and oxidation current were optimised. Under optimal experimental conditions, linear response of histamine was observed in the range 2–100 mg/l with achieved limit of detection for 0.27 mg/l of histamine. Developed method was applied for histamine determination in fermented sausages. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
